Journal: Current Protocols

Article Title: Analysis of Reactive Oxygen Species–Induced Cellular Damage in Cervical Cancer

doi: 10.1002/cpz1.70328

Figure Lengend Snippet: PlasDIC microscopy images (40× magnification) of HeLa cells after 72 hr incubation with ( A ) medium control, ( B ) solvent control (1% ethanol), ( C ) 10 µM 25(OH)D 3 , or ( D ) 250 µM calcipotriol.

Article Snippet: HeLa cells (ATCC, cat. no. CCL‐2) Complete DMEM (see recipe) 1× PBS, pH 7.4 (see recipe) .25% trypsin‐EDTA (Sigma‐Aldrich, cat. no. T4049) .4% Cytiva‐LM‐trypan blue (Separations, cat. no. SV30084.01) 10 μM 25(OH)D 3 (see recipe) 25 mM calcipotriol (see recipe) Ethanol, ACS grade, 96%, without additive (Sigma‐Aldrich, cat. no. 02870) 1 mg/ml actinomycin D (Sigma‐Aldrich, cat. no. D8418) in DMSO T25 tissue culture flasks with vent screw caps (Separations, cat. no. TPP‐90025) Humidified 37°C, 5% CO 2 incubator (water jacket type; Astec Bio, model WCl‐165) Microscope (Zeiss Axiovert 5, Carl Zeiss Microscopy) Counting chambers Neubauer improved, bright‐lined (Separations, cat. no. 810_02_04) 6‐well clear, flat‐bottom, polystyrene tissue culture plates (Lasec, cat. no. P1PLA044C‐000006)

Techniques: Microscopy, Incubation, Control, Solvent

Journal: Current Protocols

Article Title: Analysis of Reactive Oxygen Species–Induced Cellular Damage in Cervical Cancer

doi: 10.1002/cpz1.70328

Figure Lengend Snippet: Transmission electron microscopy images of cellular and mitochondrial structure. HeLa cells were treated for 72 hr with ( A , E ) medium, ( B , F ) solvent (1% ethanol), ( C , G ) 10 µM 25(OH)D 3 , or ( D , H ) 250 µM calcipotriol. Scale bar: 2 µm ( A ‐ D ), 200 nm ( E , F , H ), 500 nm ( G ).

Article Snippet: HeLa cells (ATCC, cat. no. CCL‐2) Complete DMEM (see recipe) 1× PBS, pH 7.4 (see recipe) .25% trypsin‐EDTA (Sigma‐Aldrich, cat. no. T4049) .4% Cytiva‐LM‐trypan blue (Separations, cat. no. SV30084.01) 10 μM 25(OH)D 3 (see recipe) 25 mM calcipotriol (see recipe) Ethanol, ACS grade, 96%, without additive (Sigma‐Aldrich, cat. no. 02870) 1 mg/ml actinomycin D (Sigma‐Aldrich, cat. no. D8418) in DMSO T25 tissue culture flasks with vent screw caps (Separations, cat. no. TPP‐90025) Humidified 37°C, 5% CO 2 incubator (water jacket type; Astec Bio, model WCl‐165) Microscope (Zeiss Axiovert 5, Carl Zeiss Microscopy) Counting chambers Neubauer improved, bright‐lined (Separations, cat. no. 810_02_04) 6‐well clear, flat‐bottom, polystyrene tissue culture plates (Lasec, cat. no. P1PLA044C‐000006)

Techniques: Transmission Assay, Electron Microscopy, Solvent

Journal: Current Protocols

Article Title: Analysis of Reactive Oxygen Species–Induced Cellular Damage in Cervical Cancer

doi: 10.1002/cpz1.70328

Figure Lengend Snippet: Bar graph showing relative protein expression of AKT in HeLa cells. Protein levels were normalized to the loading control (β‐actin) and expressed relative to the solvent control. Corresponding immunoblots illustrate band patterns across treatment groups. Error bars represent the mean ± standard error of the mean across three passages of cells. p < .05 was considered significant. **** p < .0001.

Article Snippet: HeLa cells (ATCC, cat. no. CCL‐2) Complete DMEM (see recipe) 1× PBS, pH 7.4 (see recipe) .25% trypsin‐EDTA (Sigma‐Aldrich, cat. no. T4049) .4% Cytiva‐LM‐trypan blue (Separations, cat. no. SV30084.01) 10 μM 25(OH)D 3 (see recipe) 25 mM calcipotriol (see recipe) Ethanol, ACS grade, 96%, without additive (Sigma‐Aldrich, cat. no. 02870) 1 mg/ml actinomycin D (Sigma‐Aldrich, cat. no. D8418) in DMSO T25 tissue culture flasks with vent screw caps (Separations, cat. no. TPP‐90025) Humidified 37°C, 5% CO 2 incubator (water jacket type; Astec Bio, model WCl‐165) Microscope (Zeiss Axiovert 5, Carl Zeiss Microscopy) Counting chambers Neubauer improved, bright‐lined (Separations, cat. no. 810_02_04) 6‐well clear, flat‐bottom, polystyrene tissue culture plates (Lasec, cat. no. P1PLA044C‐000006)

Techniques: Expressing, Control, Solvent, Western Blot